Better Understanding of How to Use the Immune Frame

The purpose of the Pre-Therapy Immune Frame is to:

1. Provide a pre cellular RGCC therapy baseline
2. Screen for high markers that could disqualify the person from being able to appropriately respond to a cellular therapy.

A pre-Immune Frame should be ordered within three months of ordering a cellular therapy. You will also need a CTC test within that time frame.

For the Immune Frame, the lab utilizes two techniques: Elisa and Flow cytometry. The percentages are based on the reference ranges of healthy/normal levels of cytokines. These levels are not qualitative, and are only semi-quantitative.

The Immune Frame is designed specifically for pre and post RGCC cellular therapy evaluation purposes only. It is not a comprehensive look at the patient’s immune system.

If there are some undesirable numbers on this test (high CD 25, CTLA 4 at any percentage or high TNF-a) it will allow time to improve the numbers before ordering a cellular therapy or while you’re waiting on the first cellular therapy to be shipped for administration. Please note that IL-6 can be a good cytokine for RGCC therapies and you want it to be high (similar to IL-2 and IL-4),

A post-therapy Immune Frame is to monitor the memory cells. These should be ordered every six months.

Below is a sample of an average pre/baseline Immune Frame:

As you can see on the above sample, the person has zero Tregs and a TNF-a of 3.41%. Although the TNF-a is elevated, it is still under 5% so the person is acceptable for a cellular therapy. However, it should be noted that this patient would still benefit from intervention to lower that marker as outlined below.

You can recommend various nutrients to help improve the results on the Immune Frame with a focus on decreasing the undesirable TNF-a which can ultimately (hopefully) decrease the Tregs.

High-TNF-a will usually respond to:

• ValAsta
• Paw Paw
• Ascorbic Acid
• Double Helix Water
• Curcumin/Turmeric
• EGCG/Green Tea
• Quercetin
• Magnesium
• Melatonin
• Vitamin E (alpha Tocopherol)
• Resveratrol
• Ashwagandha
• Citrus Flavonoids
• Genistein
• Korean Ginseng
• EPA fish oil
• Milk Thistle
• Vitamin D3
• Tart Cherry Juice
• CoQ10

Progressive muscle relaxation (PMR) which is a relaxation technique can be one of the most powerful ways to control TNF-a levels.

High-Tregs – will usually respond to:

Natural Substances:

• Curcumin
• Probiotics: 
  • B. Infantis
  • S. Boulardii
  • L. Casei
  • L. Reutari
  • L. Salivarius
  • Bifidobacterium Bifidum
  • Lactobacillus acidophilus
  • VSL#3
• Flavonoids
• Resveratrol
• Fish Oil
• Ginger
• Vitamin D3 (emulsion or micellized form)

Diet: Gluten free diet

Medications: (Generally only used by Oncologists)

• Denileukin diftitox (ONTAK)
• Daclizumab

See in-depth article on the influence of diet here:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3276397/

Important: The exception to the above are the substances that are directly cytotoxic like certain  conventional medications or natural substances such as Curcumin, Paw Paw and Ozone. You will need to do your own research as to which substances are cytotoxic or not. Cytotoxic substances should be avoided for two weeks prior to the therapy infusion and for a minimum of 21 days after the therapy has been administered.

Lifestyle Factors that can decrease immune function:

• Diet: high glycemic index foods, high fat diet, coffee, excessive alcohol
• Lifestyle: chronic insomnia, obesity, smoking, chronic stress
• Health: chronic viruses and infections (including Lyme disease)

Lifestyle Factors that can increase immune response:

• Diet: calorie restriction, Mediterranean diet
• Lifestyle: positive emotions, meditation
• Health: healthy blood sugar levels, exercise, elimination of bacterial infections, decrease of inflammation

Go here for an interesting and in-depth article on Onco Immunology here:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3382865/

You want the following to be as HIGH as possible:

• INF-γ (interferon gamma) - does well with homeopathic Citomix (From GUNA)
• IL-2 and IL-4 - these will usually respond just by down regulating TNF-a and Tregs
• IL-2 can be modulated with Melatonin, plant sterols, turkey tail, Taurine, L-Carnitine or CLA.
• IL-6 will usually respond to:
  • Mushroom extracts
  • Vitamin C
  • Vitamin D3
  • Choline (if deficient)
  • Zinc (if deficient)
  • Magnesium
  • Resveratrol
  • Melatonin
  • Guna IL- IL-2, IL-4, IL-6, IL-12, INF-Y

This is done by increasing cell-mediated immunity and humoral immunity:

• Increasing INF-γ (interferon gamma)
• Increasing IL-2 (Interleukin 2)
• Increasing IL-4 (Interleukin 4)
• Increasing IL-6 (Interleukin 6)

This is done by decreasing the inflammation markers (the lower the negative the better):

• Lowering high TNF-a (Tumor Necrosis Factor alpha)
• Lowering high Tregs (T regulatory cells) CD25 and CTLA4

Go here for a good article that highlights the effect of IL-6 in humoral immunity activation.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196041/

The goal is to develop a long-term immune response:

• T&B cells should be steadily present
• CD 80 should be over 15% and CD 86 should be over 10%
• CD 28 – B & T line should be above 0 (even barely is ok)

Sample of a successful post cellular therapy Immune Frame:

CD80 should be >15 and CD 86 should be> 10-12%. You are aiming for >22 in the final stage for optimal benefit (Note: you may not see that number until your first 6 month follow up) 

CD28 >In the beginning any number is good. and it should gradually increase to (2.0-3.5). On this
sample the CD 28 is 2.17 which is a very good number.

Summary: As the TNF-α and Tregs (CD25, CTLA4) go lower the better for the patient and the (IL-6, IL-4, IL-2, CD28, CD80, CD86] should go higher. This means the therapy is working. If the numbers of CD28 (>0.5) and the CD80, CD86 are in the >10-15 range then the therapy worked and these will likely continue to increase even more until the ultimate goal of >22

After completion of the administrations schedule of the cellular therapy, plan and schedule the mandatory follow up Immune Frame and Oncotrace tests on the following specific days:

• VACCINE PREP – Day 72
• Dendritic Cell Therapy – Day 63
• VAXO-Q-RE – Day 120 and Day 210

The above referenced mandatory follow up tests are included with the initial cost of the therapy.

If the numbers of CD28 (>0.5) and the CD80 is in the >15 range and the CD86 is in the >10 range then the therapy worked and these will likely continue to increase even more until the ultimate goal of >22.

Follow up Immune Frames are recommended at 6-month intervals to confirm that the patient is holding onto the memory cells that were developed. These are not included within the original therapy cost but they are very important.

On the 6-month follow up you’ll be looking for stable or small increases in (IL-6, IL-4, IL-2, CD28, CD80, CD86], and stable or continued decreases in TNF-α and Tregs (CD25, CTLA4). These parameters will apply to every 6-month follow up Immune Frame, hopefully for years or the rest of the recipient’s life.

A person may need a repeat of the therapy. This happens when the cancer mutates and changes the target of the original successful therapy. This could happen within a year or two or even longer. This is why the additional follow up Immune Frames are critical.

A repeat of the therapy will be required for a new target. This will require a new blood sample and the patient will incur the standard cost of the therapy. In the rare cases where the numbers do not meet the threshold even after doing all that can be done (immune building nutrients, avoidance of immune
suppressive treatments, lifestyle adjustment, stress management, booster, etc.) then the therapy is likely not going to result in achieving maximum benefit. Please note that even a partial response is still helpful to the patient.