The OncoTrail test by RGCC is a specialized diagnostic tool designed for use when the primary tumor origin is already known. It provides detailed insights for specific cancer types, serving as both a baseline assessment and a follow-up tool to monitor treatment effectiveness and detect recurrence. Here’s how it works and what it offers:

How OncoTrail Works

1. Blood Sample Collection
   • A simple blood draw is used to obtain the sample, making the process non-invasive and convenient.
2. Detection of Circulating Tumor Cells (CTCs)
   • The test identifies the presence and quantity of CTCs, which are cancer cells that have entered the bloodstream. This measurement helps gauge the cancer's activity level.
3. Biomarker Analysis for Specific Cancer Types
   • The OncoTrail test is tailored to specific cancers, offering an expanded panel of biomarkers compared to the OncoTrace test. It provides deeper insights into cancers of the:
     • Breast
     • Colon
     • Gastrointestinal (GI) tract
     • Lung
     • Melanoma
     • Prostate
     • Sarcoma
4. Phenotype and Stemness Marker Identification
   • The test evaluates:
     • Phenotype markers to confirm the cancer’s tissue origin.
     • Stemness markers to assess the cancer’s potential for aggressiveness, metastasis, treatment resistance, and recurrence.

Key Applications of OncoTrail

1. Baseline and Follow-Up Monitoring
   • Establishes a starting point for understanding the cancer’s activity and is ideal for monitoring treatment effectiveness over time.
2. Post-OncoTrace Follow-Up
   • Once it is confirmed there are no secondary cancers present, OncoTrail offers more precise tracking for the known primary cancer.
3. Long-Term Monitoring
   • Provides ongoing surveillance following successful treatment, enabling early detection of recurrence before clinical symptoms develop.

When to Use OncoTrail

• Known Primary Tumor Origin: Ideal for cases where the primary cancer type has already been identified.
• Specific Cancer Types: Particularly suited for breast, colon, GI, lung, melanoma, prostate, and sarcoma cancers.
• Post-OncoTrace: Offers refined insights for patients previously tested with OncoTrace who are now focusing on the primary tumor.

Key Benefits of OncoTrail

1. Tailored to Specific Cancers: Includes an expanded biomarker panel for precise monitoring of specific cancer types.
2. Non-Invasive: Requires only a blood sample, avoiding the need for invasive biopsies.
3. Treatment Monitoring: Tracks how well therapies are working by monitoring CTC levels and activity.
4. Early Recurrence Detection: Provides peace of mind through ongoing surveillance, catching recurrence early.
5. Personalized Prognosis: Stemness and phenotype markers provide critical insights into cancer aggressiveness and progression risks.

The OncoTrail test is a powerful tool for personalized cancer care, offering precision and clarity for patients with known primary cancers. By providing detailed insights into cancer activity and progression, it helps guide treatment decisions, improve outcomes, and deliver peace of mind.